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08/22/2024

An automated workflow based on data independent acquisition for practical and high-throughput personalized assay development and MRD monitoring in MM patients - CCLM 2024

Charissa Wijnands, Gad Armony, Somayya Noori, Jolein Gloerich, Vincent Bonifay, Hélène Caillon, Theo M. Luider, Sven Brehmer, Lennard Pfennig, Tharan Srikumar, Dennis Trede, Gary Kruppa, Thomas Dejoie, Martijn M. van Duijn, Alain J. van Gool, Joannes F.M. Jacobs and Hans J.C.T. Wessels

Clinical Chemistry and Laboratory Medicine (CCLM), 2024. https://doi.org/10.1515/cclm-2024-0306.
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An automated workflow based on data independent acquisition for practical and high-throughput personalized assay development and minimal residual disease monitoring in multiple myeloma patients

This study aimed to streamline the MS-MRD process using automated data analysis and dia-PASEF technology. By developing a workflow to identify unique patient-specific protein targets and quantify M-protein levels, the analysis achieved comparable results to PRM methods. Importantly, the new approach significantly reduced assay development time through multiplexing, bringing MS-MRD into clinical implementation.

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